Quid or fluid appearance is visible. Bacteria and background don’t have a much of difference in color. Although, bacteria are defined and visible noted. Most of the time they are single round cells. In direct stain with crystal violet, again background is colorless and glossy. Bacteria is blue and some uneven grouping is noted. In this example the dye methyl blue stain is used.
In indirect stain with Congo red stain, background is again colorless and glossy. Bacteria are orange to red and fine defined. At smaller magnified example they are grouping in uneven clusters, but in highest magnifier we can see single bacteria dark cell. B: On each examples bacterial morphologies are visible and easy identified. In the wet mount example with yeast, Cisco is noted, while is hard to determine in cheek smears. Check smear and yeast smear contain Cisco with direct staining. At direct staining plaque smear contain Cisco and bacillus.
Yeast with indirect staining contains Cisco as well. On same picture bacteria are singled while on some pictures bacteria are in group and uneven clusters. Different type of stain plays role to differentiate the shape of bacteria. C: When we are talking about difference between direct and indirect stains, main importance is acidity of the dye. Dye that we are using in direct staining is basic. The colored part of this dye is positive charge, but when in contact with negative cytoplasm of bacteria, at that point bacteria change color and become stained with purple hue.
For direct staining we are sing crystal violet or methyl blue stain. For indirect staining we are using dyes that are acidic. Usually we are using Congo red, engrossing or India ink. The acidic dye contains negatively charged color portion. When applied and in contact with negatively charged cytoplasm of bacteria it effects area around bacteria and change color into glossy or colorless. The yeast smears are very similar in all staining method, but is more visible in slides when directly stained. D: In slides with yeast smear, Cisco is notable. They are in grouped in uneven clusters and also well defined.
There are Cisco in plaque smear, but less visible and defined and scattered. Yeast smear don’t contain long bacillus, while plaque smear contains them. E: The cheek smear cells have irregular borders, and they are mostly round in the shape. Some of them are visible single cells and some are clustered and grouped unevenly. The shape of the cells are very similar as epithelial cells covering our mouth.